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Technique for measuring 50% end points in cytotoxicity assays for Clostridium difficile toxins.

Serial dilutions of Clostridium difficile culture filtrates were incubated overnight with HeLa cell monolayers. Cells were fixed in formalin, stained with crystal violet, rinsed, and drained. Cell rounding could be observed microscopically in the stained monolayers. Absorbance of the retained dye on...

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Bibliografske podrobnosti
izdano v:J Clin Pathol
Glavni avtor: Rothman, S W
Format: Artigo
Jezik:Inglês
Izdano: BMJ Publishing Group 1986
Teme:
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC499990/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3722420/
https://ncbi.nlm.nih.govhttps://doi.org/10.1136/jcp.39.6.672
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