Technique for measuring 50% end points in cytotoxicity assays for Clostridium difficile toxins.
Serial dilutions of Clostridium difficile culture filtrates were incubated overnight with HeLa cell monolayers. Cells were fixed in formalin, stained with crystal violet, rinsed, and drained. Cell rounding could be observed microscopically in the stained monolayers. Absorbance of the retained dye on...
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| Publicado no: | J Clin Pathol |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BMJ Publishing Group
1986
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC499990/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3722420/ https://ncbi.nlm.nih.govhttps://doi.org/10.1136/jcp.39.6.672 |
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