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Technique for measuring 50% end points in cytotoxicity assays for Clostridium difficile toxins.

Serial dilutions of Clostridium difficile culture filtrates were incubated overnight with HeLa cell monolayers. Cells were fixed in formalin, stained with crystal violet, rinsed, and drained. Cell rounding could be observed microscopically in the stained monolayers. Absorbance of the retained dye on...

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Détails bibliographiques
Auteur principal: Rothman, S W
Format: Artigo
Langue:Inglês
Publié: 1986
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC499990/
https://ncbi.nlm.nih.gov/pubmed/3722420
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