Multiplex gene editing via CRISPR/Cas9 exhibits desirable muscle hypertrophy without detectable off-target effects in sheep

The CRISPR/Cas9 system provides a flexible approach for genome engineering of genetic loci. Here, we successfully achieved precise gene targeting in sheep by co-injecting one-cell-stage embryos with Cas9 mRNA and RNA guides targeting three genes (MSTN, ASIP, and BCO2). We carefully examined the sgRN...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Wang, Xiaolong, Niu, Yiyuan, Zhou, Jiankui, Yu, Honghao, Kou, Qifang, Lei, Anmin, Zhao, Xiaoe, Yan, Hailong, Cai, Bei, Shen, Qiaoyan, Zhou, Shiwei, Zhu, Haijing, Zhou, Guangxian, Niu, Wenzhi, Hua, Jinlian, Jiang, Yu, Huang, Xingxu, Ma, Baohua, Chen, Yulin
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4999810/
https://ncbi.nlm.nih.gov/pubmed/27562433
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep32271
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