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Unbiased Deep Sequencing of RNA Viruses from Clinical Samples
Here we outline a next-generation RNA sequencing protocol that enables de novo assemblies and intra-host variant calls of viral genomes collected from clinical and biological sources. The method is unbiased and universal; it uses random primers for cDNA synthesis and requires no prior knowledge of t...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4993327/ https://ncbi.nlm.nih.gov/pubmed/27403729 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54117 |
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