Preparing Unbiased T-Cell Receptor and Antibody cDNA Libraries for the Deep Next Generation Sequencing Profiling

High-throughput sequencing has the power to reveal the nature of adaptive immunity as represented by the full complexity of T-cell receptor (TCR) and antibody (IG) repertoires, but is at present severely compromised by the quantitative bias, bottlenecks, and accumulated errors that inevitably occur...

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Detalhes bibliográficos
Main Authors: Mamedov, Ilgar Z., Britanova, Olga V., Zvyagin, Ivan V., Turchaninova, Maria A., Bolotin, Dmitriy A., Putintseva, Ekaterina V., Lebedev, Yuriy B., Chudakov, Dmitriy M.
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2013
Assuntos:
Immunology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3870325/
https://ncbi.nlm.nih.gov/pubmed/24391640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fimmu.2013.00456
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