Enhanced methods for unbiased deep sequencing of Lassa and Ebola RNA viruses from clinical and biological samples

We have developed a robust RNA sequencing method for generating complete de novo assemblies with intra-host variant calls of Lassa and Ebola virus genomes in clinical and biological samples. Our method uses targeted RNase H-based digestion to remove contaminating poly(rA) carrier and ribosomal RNA....

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Bibliografiske detaljer
Udgivet i:Genome Biol
Main Authors: Matranga, Christian B, Andersen, Kristian G, Winnicki, Sarah, Busby, Michele, Gladden, Adrianne D, Tewhey, Ryan, Stremlau, Matthew, Berlin, Aaron, Gire, Stephen K, England, Eleina, Moses, Lina M, Mikkelsen, Tarjei S, Odia, Ikponmwonsa, Ehiane, Philomena E, Folarin, Onikepe, Goba, Augustine, Kahn, S Humarr, Grant, Donald S, Honko, Anna, Hensley, Lisa, Happi, Christian, Garry, Robert F, Malboeuf, Christine M, Birren, Bruce W, Gnirke, Andreas, Levin, Joshua Z, Sabeti, Pardis C
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2014
Fag:
Method
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4262991/
https://ncbi.nlm.nih.gov/pubmed/25403361
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-014-0519-7
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