Enhanced methods for unbiased deep sequencing of Lassa and Ebola RNA viruses from clinical and biological samples

We have developed a robust RNA sequencing method for generating complete de novo assemblies with intra-host variant calls of Lassa and Ebola virus genomes in clinical and biological samples. Our method uses targeted RNase H-based digestion to remove contaminating poly(rA) carrier and ribosomal RNA....

Descripció completa

Guardat en:
Dades bibliogràfiques
Publicat a:Genome Biol
Autors principals: Matranga, Christian B, Andersen, Kristian G, Winnicki, Sarah, Busby, Michele, Gladden, Adrianne D, Tewhey, Ryan, Stremlau, Matthew, Berlin, Aaron, Gire, Stephen K, England, Eleina, Moses, Lina M, Mikkelsen, Tarjei S, Odia, Ikponmwonsa, Ehiane, Philomena E, Folarin, Onikepe, Goba, Augustine, Kahn, S Humarr, Grant, Donald S, Honko, Anna, Hensley, Lisa, Happi, Christian, Garry, Robert F, Malboeuf, Christine M, Birren, Bruce W, Gnirke, Andreas, Levin, Joshua Z, Sabeti, Pardis C
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2014
Matèries:
Method
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4262991/
https://ncbi.nlm.nih.gov/pubmed/25403361
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-014-0519-7
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!

Ítems similars