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Use of a new proximity assay (NanoBRET) to investigate the ligand‐binding characteristics of three fluorescent ligands to the human β (1)‐adrenoceptor expressed in HEK‐293 cells

Previous research has indicated that allosteric interactions across the dimer interface of β (1)‐adrenoceptors may be responsible for a secondary low affinity binding conformation. Here we have investigated the potential for probe dependence, in the determination of antagonist pK(i) values at the hu...

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Библиографические подробности
Опубликовано в: :Pharmacol Res Perspect
Главные авторы: Soave, Mark, Stoddart, Leigh A., Brown, Alastair, Woolard, Jeanette, Hill, Stephen J.
Формат: Artigo
Язык:Inglês
Опубликовано: John Wiley and Sons Inc. 2016
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4988514/
https://ncbi.nlm.nih.gov/pubmed/27588207
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/prp2.250
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