Tn10 insertion specificity is strongly dependent upon sequences immediately adjacent to the target-site consensus sequence.
Transposon Tn10 inserts preferentially into particular "hotspots" that have been shown by sequence analysis to contain the symmetrical consensus sequence 5'-GCTNAGC-3'. This consensus is necessary but not sufficient to determine insertion specificity. We have mutagenized a known hotspot to identify...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1992
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC49842/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1325639/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.17.7996 |
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