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Optimization of High-Throughput Sequencing Kinetics for determining enzymatic rate constants of thousands of RNA substrates

Quantification of the specificity of RNA binding proteins and RNA processing enzymes is essential to understanding their fundamental roles in biological processes. High Throughput Sequencing Kinetics (HTS-Kin) uses high throughput sequencing and internal competition kinetics to simultaneously monito...

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Detalhes bibliográficos
Publicado no:Anal Biochem
Main Authors: Niland, Courtney N., Jankowsky, Eckhard, Harris, Michael E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4980219/
https://ncbi.nlm.nih.gov/pubmed/27296633
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2016.06.004
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