Dual Labeling Biotin Switch Assay to Reduce Bias Derived from Different Cysteine Subpopulations: A Method to Maximize S-Nitrosylation Detection

RATIONALE: S-nitrosylation (SNO), an oxidative post-translational modification of cysteine residues, responds to changes in the cardiac redox-environment. Classic biotin switch assay and its derivatives are the most common methods used for detecting SNO. In this approach, the labile SNO group is sel...

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Udgivet i:Circ Res
Main Authors: Chung, Heaseung Sophia, Murray, Christopher I., Venkatraman, Vidya, Crowgey, Erin L., Rainer, Peter P., Cole, Robert N., Bomgarden, Ryan D., Rogers, John C., Balkan, Wayne, Hare, Joshua M., Kass, David A., Van Eyk, Jennifer E.
Format: Artigo
Sprog:Inglês
Udgivet: 2015
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4979977/
https://ncbi.nlm.nih.gov/pubmed/26338901
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1161/CIRCRESAHA.115.307336
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