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An assay for 26S proteasome activity based on fluorescence anisotropy measurements of dye-labeled protein substrates

The 26S proteasome is the molecular machine at the center of the ubiquitin–proteasome system and is responsible for adjusting the concentrations of many cellular proteins. It is a drug target in several human diseases, and assays for the characterization of modulators of its activity are valuable. T...

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Détails bibliographiques
Publié dans:Anal Biochem
Auteurs principaux: Bhattacharyya, Sucharita, Renn, Jonathan P., Yu, Houqing, Marko, John F., Matouschek, Andreas
Format: Artigo
Langue:Inglês
Publié: 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4976823/
https://ncbi.nlm.nih.gov/pubmed/27296635
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2016.05.026
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