Diisopropylethylamine/hexafluoroisopropanol-mediated ion-pairing UHPLC-MS for phosphate and carboxylate metabolite analysis: utility for studying cellular metabolism

RATIONALE: Mass spectrometric (MS) analysis of low molecular weight polar metabolites can be challenging because of poor chromatographic resolution of isomers and insufficient ionization efficiency. These metabolites include intermediates in key metabolic pathways, such as glycolysis, the pentose ph...

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Bibliografische gegevens
Gepubliceerd in:Rapid Commun Mass Spectrom
Hoofdauteurs: Guo, Lili, Worth, Andrew J., Mesaros, Clementina, Snyder, Nathaniel W., Glickson, Jerry D., Blair, Ian A.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2016
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4970762/
https://ncbi.nlm.nih.gov/pubmed/27476658
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/rcm.7667
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