PFA fixation enables artifact-free super-resolution imaging of the actin cytoskeleton and associated proteins

Super-resolution microscopy (SRM) allows precise localization of proteins in cellular organelles and structures, including the actin cytoskeleton. Yet sample preparation protocols for SRM are rather anecdotal and still being optimized. Thus, SRM-based imaging of the actin cytoskeleton and associated...

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Bibliografische gegevens
Gepubliceerd in:Biol Open
Hoofdauteurs: Leyton-Puig, Daniela, Kedziora, Katarzyna M., Isogai, Tadamoto, van den Broek, Bram, Jalink, Kees, Innocenti, Metello
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: The Company of Biologists Ltd 2016
Onderwerpen:
Methods & Techniques
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4958280/
https://ncbi.nlm.nih.gov/pubmed/27378434
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/bio.019570
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