Combined in vitro transcription and reverse transcription to amplify and label complex synthetic oligonucleotide probe libraries

Oligonucleotide microarrays allow the production of complex custom oligonucleotide libraries for nucleic acid detection–based applications such as fluorescence in situ hybridization (FISH). We have developed a PCR-free method to make single-stranded DNA (ssDNA) fluorescent probes through an intermed...

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Vydáno v:Biotechniques
Hlavní autoři: Murgha, Yusuf, Beliveau, Brian, Semrau, Kassandra, Schwartz, Donald, Wu, Chao-ting, Gulari, Erdogan, Rouillard, Jean-Marie
Médium: Artigo
Jazyk:Inglês
Vydáno: 2015
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4949592/
https://ncbi.nlm.nih.gov/pubmed/26054766
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114298
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