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Combined in vitro transcription and reverse transcription to amplify and label complex synthetic oligonucleotide probe libraries
Oligonucleotide microarrays allow the production of complex custom oligonucleotide libraries for nucleic acid detection–based applications such as fluorescence in situ hybridization (FISH). We have developed a PCR-free method to make single-stranded DNA (ssDNA) fluorescent probes through an intermed...
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| Pubblicato in: | Biotechniques |
|---|---|
| Autori principali: | , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2015
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4949592/ https://ncbi.nlm.nih.gov/pubmed/26054766 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114298 |
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