Combined in vitro transcription and reverse transcription to amplify and label complex synthetic oligonucleotide probe libraries

Oligonucleotide microarrays allow the production of complex custom oligonucleotide libraries for nucleic acid detection–based applications such as fluorescence in situ hybridization (FISH). We have developed a PCR-free method to make single-stranded DNA (ssDNA) fluorescent probes through an intermed...

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Bibliographische Detailangaben
Veröffentlicht in:Biotechniques
Hauptverfasser: Murgha, Yusuf, Beliveau, Brian, Semrau, Kassandra, Schwartz, Donald, Wu, Chao-ting, Gulari, Erdogan, Rouillard, Jean-Marie
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2015
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4949592/
https://ncbi.nlm.nih.gov/pubmed/26054766
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114298
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