Mass spectrometry locates local and allosteric conformational changes that occur on cofactor binding

Fdc1 is a decarboxylase enzyme that requires the novel prenylated FMN cofactor for activity. Here, we use it as an exemplar system to show how native top-down and bottom-up mass spectrometry can measure the structural effect of cofactor binding by a protein. For Fdc1(Ubix), the cofactor confers stru...

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Vydáno v:Nat Commun
Hlavní autoři: Beveridge, Rebecca, Migas, Lukasz G., Payne, Karl A. P., Scrutton, Nigel S., Leys, David, Barran, Perdita E.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group 2016
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4947166/
https://ncbi.nlm.nih.gov/pubmed/27418477
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms12163
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