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Reconstitution of selective HIV-1 RNA packaging in vitro by membrane-bound Gag assemblies

HIV-1 Gag selects and packages a dimeric, unspliced viral RNA in the context of a large excess of cytosolic human RNAs. As Gag assembles on the plasma membrane, the HIV-1 genome is enriched relative to cellular RNAs by an unknown mechanism. We used a minimal system consisting of purified RNAs, recom...

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Bibliographic Details
Published in:eLife
Main Authors: Carlson, Lars-Anders, Bai, Yun, Keane, Sarah C, Doudna, Jennifer A, Hurley, James H
Format: Artigo
Language:Inglês
Published: eLife Sciences Publications, Ltd 2016
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4946900/
https://ncbi.nlm.nih.gov/pubmed/27343348
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.14663
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