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Using ApoE-Nanolipoprotein Particles to Analyze SNARE-Induced Fusion Pores

Here we introduce ApoE-based Nanolipoprotein particles (NLP) – soluble, discoidal bilayer mimetic of ~23 nm in diameter, as fusion partners to study the dynamics of fusion pores induced by SNARE proteins. Using in vitro lipid mixing and content release assays, we report that NLPs reconstituted with...

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Bibliographic Details
Published in:Langmuir
Main Authors: Bello, Oscar D., Auclair, Sarah M., Rothman, James E., Krishnakumar, Shyam S.
Format: Artigo
Language:Inglês
Published: 2016
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4946868/
https://ncbi.nlm.nih.gov/pubmed/26972604
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.langmuir.6b00245
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