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Methodology for Quantitative Characterization of Fluorophore Photoswitching to Predict Superresolution Microscopy Image Quality

Single-molecule localization microscopy (SMLM) image quality and resolution strongly depend on the photoswitching properties of fluorophores used for sample labeling. Development of fluorophores with optimized photoswitching will considerably improve SMLM spatial and spectral resolution. Currently,...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Bittel, Amy M., Nickerson, Andrew, Saldivar, Isaac S., Dolman, Nick J., Nan, Xiaolin, Gibbs, Summer L.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4944197/
https://ncbi.nlm.nih.gov/pubmed/27412307
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep29687
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