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Methodology for Quantitative Characterization of Fluorophore Photoswitching to Predict Superresolution Microscopy Image Quality

Single-molecule localization microscopy (SMLM) image quality and resolution strongly depend on the photoswitching properties of fluorophores used for sample labeling. Development of fluorophores with optimized photoswitching will considerably improve SMLM spatial and spectral resolution. Currently,...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Bittel, Amy M., Nickerson, Andrew, Saldivar, Isaac S., Dolman, Nick J., Nan, Xiaolin, Gibbs, Summer L.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4944197/
https://ncbi.nlm.nih.gov/pubmed/27412307
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep29687
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