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Methodology for Quantitative Characterization of Fluorophore Photoswitching to Predict Superresolution Microscopy Image Quality

Single-molecule localization microscopy (SMLM) image quality and resolution strongly depend on the photoswitching properties of fluorophores used for sample labeling. Development of fluorophores with optimized photoswitching will considerably improve SMLM spatial and spectral resolution. Currently,...

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Detalles Bibliográficos
Publicado en:Sci Rep
Autores principales: Bittel, Amy M., Nickerson, Andrew, Saldivar, Isaac S., Dolman, Nick J., Nan, Xiaolin, Gibbs, Summer L.
Formato: Artigo
Lenguaje:Inglês
Publicado: Nature Publishing Group 2016
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4944197/
https://ncbi.nlm.nih.gov/pubmed/27412307
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep29687
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