Global Membrane Protein Interactome Analysis using In vivo Crosslinking and Mass Spectrometry-based Protein Correlation Profiling

We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility du...

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Veröffentlicht in:Mol Cell Proteomics
Hauptverfasser: Larance, Mark, Kirkwood, Kathryn J., Tinti, Michele, Brenes Murillo, Alejandro, Ferguson, Michael A. J., Lamond, Angus I.
Format: Artigo
Sprache:Inglês
Veröffentlicht: The American Society for Biochemistry and Molecular Biology 2016
Schlagworte:
Technological Innovation and Resources
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4937518/
https://ncbi.nlm.nih.gov/pubmed/27114452
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O115.055467
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