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Probing the Conformation of the ISWI ATPase Domain With Genetically Encoded Photoreactive Crosslinkers and Mass Spectrometry

We present a strategy for rapidly gaining structural information about a protein from crosslinks formed by genetically encoded unnatural amino acids. We applied it to ISWI, a chromatin remodeling enzyme involved in chromatin assembly, DNA replication and transcription. ISWI is part of the vast Snf2...

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Detalhes bibliográficos
Main Authors: Forné, Ignasi, Ludwigsen, Johanna, Imhof, Axel, Becker, Peter B., Mueller-Planitz, Felix
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3322568/
https://ncbi.nlm.nih.gov/pubmed/22167269
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M111.012088
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