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Stable transformation of a mosquito cell line results in extraordinarily high copy numbers of the plasmid.

Stable incorporation of high copy numbers (greater than 10,000 per cell) of a plasmid vector containing a gene conferring resistance to the antibiotic hygromycin was achieved in a cell line derived from the Aedes albopictus mosquito. Plasmid sequences were readily observed by ethidium bromide staini...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Monroe, T J, Muhlmann-Diaz, M C, Kovach, M J, Carlson, J O, Bedford, J S, Beaty, B J
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC49369/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1631052/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.13.5725
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