Stable transformation of a mosquito cell line results in extraordinarily high copy numbers of the plasmid.
Stable incorporation of high copy numbers (greater than 10,000 per cell) of a plasmid vector containing a gene conferring resistance to the antibiotic hygromycin was achieved in a cell line derived from the Aedes albopictus mosquito. Plasmid sequences were readily observed by ethidium bromide staini...
Guardat en:
| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1992
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC49369/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1631052/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.13.5725 |
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