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Pyridine Hemochromagen Assay for Determining the Concentration of Heme in Purified Protein Solutions

Heme is a common cofactor in proteins, found in hemoglobin, myoglobin, cytochrome P450, DGCR8, and nitric oxide synthase, among others. This protocol describes a method for quantifying heme that works best in purified protein samples. This protocol might be used to, for example, determine whether a...

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Bibliografiske detaljer
Udgivet i:Bio Protoc
Main Authors: Barr, Ian, Guo, Feng
Format: Artigo
Sprog:Inglês
Udgivet: 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4932910/
https://ncbi.nlm.nih.gov/pubmed/27390766
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