Radical SAM Catalysis via an Organometallic Intermediate with an Fe–[5′-C]-Deoxyadenosyl Bond

Radical SAM enzymes use a [4Fe-4S] cluster to cleave S-adenosylmethionine (SAM) to initiate diverse radical reactions. These are thought to involve the 5′-deoxyadenosyl radical intermediate, which has not yet been detected. Here we rapid freeze-quench trap a catalytically competent intermediate in t...

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Veröffentlicht in:Science
Hauptverfasser: Horitani, Masaki, Shisler, Krista, Broderick, William E., Hutcheson, Rachel U., Duschene, Kaitlin S., Marts, Amy R., Hoffman, Brian M., Broderick, Joan B.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2016
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4929858/
https://ncbi.nlm.nih.gov/pubmed/27174986
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.aaf5327
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