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Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques

A detailed protocol is provided here to identify amyloid Aβ plaques in brain sections from Alzheimer's disease mouse models before pre-embedding immunostaining (specifically for ionized calcium-binding adapter molecule 1 (IBA1), a calcium binding protein expressed by microglia) and tissue proce...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Bisht, Kanchan, El Hajj, Hassan, Savage, Julie C., Sánchez, Maria G., Tremblay, Marie-Ève
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2016
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4927759/
https://ncbi.nlm.nih.gov/pubmed/27286292
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54060
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