16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform

BACKGROUND: Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the community. Numerous factors, including DNA extraction method, primer seque...

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發表在:BMC Microbiol
Main Authors: Fouhy, Fiona, Clooney, Adam G., Stanton, Catherine, Claesson, Marcus J., Cotter, Paul D.
格式: Artigo
語言:Inglês
出版: BioMed Central 2016
主題:
Research Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4921037/
https://ncbi.nlm.nih.gov/pubmed/27342980
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12866-016-0738-z
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