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16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform

BACKGROUND: Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the community. Numerous factors, including DNA extraction method, primer seque...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:BMC Microbiol
Prif Awduron: Fouhy, Fiona, Clooney, Adam G., Stanton, Catherine, Claesson, Marcus J., Cotter, Paul D.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: BioMed Central 2016
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4921037/
https://ncbi.nlm.nih.gov/pubmed/27342980
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12866-016-0738-z
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