Improved binding site assignment by high-resolution mapping of RNA–protein interactions using iCLIP

Individual-nucleotide resolution crosslinking and immunoprecipitation (iCLIP) allows the determination of crosslinking sites of RNA-binding proteins (RBPs) on RNAs. iCLIP is based on ultraviolet light crosslinking of RBPs to RNA, reverse transcription and high-throughput sequencing of fragments term...

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Vydáno v:Nat Commun
Hlavní autoři: Hauer, Christian, Curk, Tomaz, Anders, Simon, Schwarzl, Thomas, Alleaume, Anne-Marie, Sieber, Jana, Hollerer, Ina, Bhuvanagiri, Madhuri, Huber, Wolfgang, Hentze, Matthias W., Kulozik, Andreas E.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group 2015
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4918375/
https://ncbi.nlm.nih.gov/pubmed/26260686
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms8921
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