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Reprogramming Caspase-7 Specificity by Regio-Specific Mutations and Selection Provides Alternate Solutions for Substrate Recognition

The ability to routinely engineer protease specificity can allow us to better understand and modulate their biology for expanded therapeutic and industrial applications. Here we report a new approach based on a caged green fluorescent protein (CA-GFP) reporter that allows for flow-cytometry-based se...

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Detalles Bibliográficos
Publicado en:ACS Chem Biol
Autores principales: Hill, Maureen E., MacPherson, Derek J., Wu, Peng, Julien, Olivier, Wells, James A., Hardy, Jeanne A.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2016
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4912419/
https://ncbi.nlm.nih.gov/pubmed/27032039
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acschembio.5b00971
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