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Reprogramming Caspase-7 Specificity by Regio-Specific Mutations and Selection Provides Alternate Solutions for Substrate Recognition

The ability to routinely engineer protease specificity can allow us to better understand and modulate their biology for expanded therapeutic and industrial applications. Here we report a new approach based on a caged green fluorescent protein (CA-GFP) reporter that allows for flow-cytometry-based se...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:ACS Chem Biol
Prif Awduron: Hill, Maureen E., MacPherson, Derek J., Wu, Peng, Julien, Olivier, Wells, James A., Hardy, Jeanne A.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2016
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4912419/
https://ncbi.nlm.nih.gov/pubmed/27032039
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acschembio.5b00971
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