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Isothermal titration calorimetry of ion-coupled membrane transporters

Binding of ligands, ranging from proteins to ions, to membrane proteins is associated with absorption or release of heat that can be detected by isothermal titration calorimetry (ITC). Such measurements not only provide binding affinities but also afford direct access to thermodynamic parameters of...

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Detalles Bibliográficos
Publicado en:Methods
Main Authors: Boudker, Olga, SeCheol, Oh
Formato: Artigo
Idioma:Inglês
Publicado: 2015
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4912014/
https://ncbi.nlm.nih.gov/pubmed/25676707
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2015.01.012
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