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Isothermal titration calorimetry of ion-coupled membrane transporters
Binding of ligands, ranging from proteins to ions, to membrane proteins is associated with absorption or release of heat that can be detected by isothermal titration calorimetry (ITC). Such measurements not only provide binding affinities but also afford direct access to thermodynamic parameters of...
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| Опубликовано в: : | Methods |
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| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2015
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4912014/ https://ncbi.nlm.nih.gov/pubmed/25676707 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2015.01.012 |
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