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Visualizing the formation of an RNA folding intermediate through a fast highly modular secondary structure switch

Intermediates play important roles in RNA folding but can be difficult to characterize when short-lived or not significantly populated. By combining (15)N relaxation dispersion NMR with chemical probing, we visualized a fast (k(ex)=k(1)+k(−1)≈423 s(−1)) secondary structural switch directed towards a...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Xue, Yi, Gracia, Brant, Herschlag, Daniel, Russell, Rick, Al-Hashimi, Hashim M.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4909931/
https://ncbi.nlm.nih.gov/pubmed/27292179
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms11768
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