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Crystal structures of two monomeric triosephosphate isomerase variants identified via a directed-evolution protocol selecting for l-arabinose isomerase activity

The crystal structures are described of two variants of A-TIM: Ma18 (2.7 Å resolution) and Ma21 (1.55 Å resolution). A-TIM is a monomeric loop-deletion variant of triosephosphate isomerase (TIM) which has lost the TIM catalytic properties. Ma18 and Ma21 were identified after extensive directed-evolu...

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Vydáno v:Acta Crystallogr F Struct Biol Commun
Hlavní autoři: Krause, Mirja, Kiema, Tiila-Riikka, Neubauer, Peter, Wierenga, Rik K.
Médium: Artigo
Jazyk:Inglês
Vydáno: International Union of Crystallography 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4909251/
https://ncbi.nlm.nih.gov/pubmed/27303904
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X16007548
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