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Design, creation, and characterization of a stable, monomeric triosephosphate isomerase.

Protein engineering on trypanosomal triosephosphate isomerase (TIM) converted this oligomeric enzyme into a stable, monomeric protein that is enzymatically active. Wild-type TIM consists of two identical subunits that form a very tight dimer involving interactions of 32 residues of each subunit. By...

詳細記述

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書誌詳細
主要な著者: Borchert, T V, Abagyan, R, Jaenicke, R, Wierenga, R K
フォーマット: Artigo
言語:Inglês
出版事項: 1994
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC43190/
https://ncbi.nlm.nih.gov/pubmed/8108439
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