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Crystal structure of an engineered subtilisin inhibitor complexed with bovine trypsin.

Proteinase specificity of a proteinaceous inhibitor of subtilisin (SSI; Streptomyces subtilisin inhibitor) can be altered so as to strongly inhibit trypsin simply by replacing P1 methionine with lysine (with or without concomitant change of the P4 residue) through site-directed mutagenesis. Now the...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Takeuchi, Y, Nonaka, T, Nakamura, K T, Kojima, S, Miura, K, Mitsui, Y
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1992
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC49091/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1584773/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.10.4407
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