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Functional domains in Fok I restriction endonuclease.

The PCR was used to alter transcriptional and translational signals surrounding the Flavobacterium okeanokoites restriction endonuclease (fokIR) gene, so as to achieve high expression in Escherichia coli. By changing the ribosome-binding site sequence preceding the fokIR gene to match the consensus...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Li, L, Wu, L P, Chandrasegaran, S
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1992
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC49064/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1584761/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.10.4275
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