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Solution NMR Experiment for Measurement of (15)N-(1)H Residual Dipolar Couplings in Large Proteins and Supramolecular Complexes

NMR residual dipolar couplings (RDCs) are exquisite probes of protein structure and dynamics. A new solution NMR experiment named 2D SE2 J-TROSY is presented to measure N-H RDCs for proteins and supramolecular complexes in excess of 200 kDa. This enables validation and refinement of their X-ray crys...

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Bibliografski detalji
Izdano u:J Am Chem Soc
Glavni autori: Eletsky, Alexander, Pulavarti, Surya V.S.R.K., Beaumont, Victor, Gollnick, Paul, Szyperski, Thomas
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4905564/
https://ncbi.nlm.nih.gov/pubmed/26293598
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.5b07010
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