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SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans

In principle, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 allows genetic tags to be inserted at any locus. However, throughput is limited by the laborious construction of repair templates and guide RNA constructs and by the identification of modified strains. We have deve...

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Detalhes bibliográficos
Publicado no:Genetics
Main Authors: Schwartz, Matthew L., Jorgensen, Erik M.
Formato: Artigo
Idioma:Inglês
Publicado em: Genetics Society of America 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4905529/
https://ncbi.nlm.nih.gov/pubmed/26837755
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.115.184275
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