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SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans

In principle, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 allows genetic tags to be inserted at any locus. However, throughput is limited by the laborious construction of repair templates and guide RNA constructs and by the identification of modified strains. We have deve...

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Vydáno v:Genetics
Hlavní autoři: Schwartz, Matthew L., Jorgensen, Erik M.
Médium: Artigo
Jazyk:Inglês
Vydáno: Genetics Society of America 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4905529/
https://ncbi.nlm.nih.gov/pubmed/26837755
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.115.184275
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