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SapTrap Assembly of Caenorhabditis elegans MosSCI Transgene Vectors
The Mos1-mediated Single-Copy Insertion (MosSCI) method is widely used to establish stable Caenorhabditis elegans transgenic strains. Cloning MosSCI targeting plasmids can be cumbersome because it requires assembling multiple genetic elements including a promoter, a 3′UTR and gene fragments. Recentl...
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| Udgivet i: | G3 (Bethesda) |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Genetics Society of America
2019
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7003106/ https://ncbi.nlm.nih.gov/pubmed/31848219 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.119.400822 |
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