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Intracavitary Ligand Distribution in Tear Lipocalin by Site-Directed Tryptophan Fluorescence
Site-directed tryptophan fluorescence has been successfully used to determine the solution structure of tear lipocalin. Here, the technique is extended to measure the binding energy landscape. Single Trp mutants of tear lipocalin are bound to the native ligand and an analogue tagged with a quencher...
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| Publicado no: | Biochemistry |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4902112/ https://ncbi.nlm.nih.gov/pubmed/19586017 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi9005557 |
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