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Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins

Fluorescence resonance energy transfer (FRET) technology has been used to develop genetically encoded fluorescent indicators for various cellular functions. Although most indicators have cyan- and yellow-emitting fluorescent proteins (CFP and YFP) as FRET donor and acceptor, their poor dynamic range...

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Main Authors: Nagai, Takeharu, Yamada, Shuichi, Tominaga, Takashi, Ichikawa, Michinori, Miyawaki, Atsushi
格式: Artigo
語言:Inglês
出版: National Academy of Sciences 2004
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC490022/
https://ncbi.nlm.nih.gov/pubmed/15247428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0400417101
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