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RNA SHAPE Analysis of Small RNAs and Riboswitches
We describe structural analysis of RNAs by SHAPE chemical probing. RNAs are treated with 1-methyl-7-nitroisatoic anhydride (1M7), a reagent that detects local nucleotides flexibility, and N-methylisatoic anhydride (NMIA) and 1-methyl-6-nitroisatoic anhydride (1M6), reagents which together detect hig...
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| Publicado en: | Methods Enzymol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2014
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4896901/ https://ncbi.nlm.nih.gov/pubmed/25432749 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-801122-5.00008-8 |
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