RNA SHAPE Analysis of Small RNAs and Riboswitches

We describe structural analysis of RNAs by SHAPE chemical probing. RNAs are treated with 1-methyl-7-nitroisatoic anhydride (1M7), a reagent that detects local nucleotides flexibility, and N-methylisatoic anhydride (NMIA) and 1-methyl-6-nitroisatoic anhydride (1M6), reagents which together detect hig...

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Detaylı Bibliyografya
Yayımlandı:Methods Enzymol
Asıl Yazarlar: Rice, Greggory M., Busan, Steven, Karabiber, Fethullah, Favorov, Oleg V., Weeks, Kevin M.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2014
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4896901/
https://ncbi.nlm.nih.gov/pubmed/25432749
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-801122-5.00008-8
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