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High Throughput Gene Complementation Screening Permits Identification of a Mammalian Mitochondrial Protein Synthesis (ρ(−)) Mutant

To identify nuclear DNA (nDNA) oxidative phosphorylation (OXPHOS) gene mutations using cultured cells, we have developed a complementation system based on retroviral transduction with a full length cDNA expression library and selection for OXHOS function by growth in galactose. We have used this sys...

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Bibliografiske detaljer
Udgivet i:Biochim Biophys Acta
Main Authors: Potluri, Prasanth, Procaccio, Vincent, Scheffler, Immo E, Wallace, Douglas C
Format: Artigo
Sprog:Inglês
Udgivet: 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4893908/
https://ncbi.nlm.nih.gov/pubmed/26946086
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbabio.2016.02.021
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