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Engineering a ribozyme cleavage-induced split fluorescent aptamer complementation assay

Hammerhead ribozymes are self-cleaving RNA molecules capable of regulating gene expression in living cells. Their cleavage performance is strongly influenced by intra-molecular loop–loop interactions, a feature not readily accessible through modern prediction algorithms. Ribozyme engineering and eff...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Autores principales: Ausländer, Simon, Fuchs, David, Hürlemann, Samuel, Ausländer, David, Fussenegger, Martin
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2016
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4889925/
https://ncbi.nlm.nih.gov/pubmed/26939886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw117
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